质粒名称: | pYPQ203 (pMDC32-Ubi1) |
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出品公司: | 爱迪基因 |
目录编号: | 86207 |
存储实验室: | Yiping Qi |
载体骨架基本信息 |
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载体名称: | pMDC32 |
载体出品公司: | -- |
载体抗性: | 氯霉素和卡那霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | 添加到pYPQ202主链的拟南芥泛素10(AtUbi10)启动子 |
载体类型: | 植物表达;网关兼容attR1-attR2目的载体 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | Maize ubiquitin 1 (ZmUbi1) |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pYPQ203 (pMDC32-Ubi1) was a gift from Yiping Qi (Addgene plasmid # 86207 ; http://n2t.net/addgene:86207 ; RRID:Addgene_86207) |
参考文献部分: | A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. 10.1038/nplants.2017.18 PubMed 28211909 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYPQ203 (pMDC32-Ubi1) 14174 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Multisite Gateway T-DNA entry plasmid (attR1 Zea mays Ubi1 promoter and Hygromiycin resistance marker. ACCESSION . VERSION . KEYWORDS pYPQ203 (pMDC32-Ubi1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14174) AUTHORS Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y TITLE A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. JOURNAL Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. PUBMED 28211909 REFERENCE 2 (bases 1 to 14174) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14174 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..26) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10..26) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(23..45) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 34..50 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(58..88) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 103..124 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 315..991 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" primer_bind 928..948 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 1058..2083 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" polyA_signal 2124..2298 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 2376..2400 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 4024..4818 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 4905..5493 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5394..5413 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5647..5664 /label=L4440 /note="L4440 vector, forward primer" misc_feature 5679..5819 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(5745..5764) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5905..5927 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 6163..6357 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(6423..7496) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(7925..8554) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 9849..9873 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 10061..10083 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 10075..10092 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 10076..10092 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 10108..12105 /label=Ubi promoter /note="maize polyubiquitin gene promoter" protein_bind 12148..12272 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 12297..12327 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 12381..13061 /codon_start=1 /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE DPAY" primer_bind complement(12447..12466) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 13381..13686 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 13565..13584 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(13727..13851) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 13915..14167 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" ORIGIN 1 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc 241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa 301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc 361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac 421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt 481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg 541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa 601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt 661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac 721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt 781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt 841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc 1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc 1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct 1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct 1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc 1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac 1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact 1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag 1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg 1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga 1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg 1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa 1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga 1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta 1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg 1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg 1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca 1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag 2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg 2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag 2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat 2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc 2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc 2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca 2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc 2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc 2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga 2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc 2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg 2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg 2761 tcttgttata attagggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct 2821 ggagccatag aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca 2881 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc 2941 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc 3001 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga 3061 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt 3121 tatccctgga tagcgccttg ccggaccgca ccaccatcat gaatttccgc cacctgctgg 3181 agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg gccgaagcag 3241 gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca cccagctcga 3301 ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt 3361 ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc 3421 tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg ctgcatggag 3481 aggagcaatt tgtctcagcc gatgccggct accaaggggc gccacagcgc gaggagctgg 3541 ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg caaggtaaga accttgaaac 3601 agcatccacg caagaacaaa acggccatca acatcgaata catgaaagcc agcatccggg 3661 ccagggtgga gcacccattt cgcatcatca agcgacagtt cggcttcgtg aaagccagat 3721 acaaggggtt gctgaaaaac gataaccaac tggcgatgtt attcacgctg gccaacctgt 3781 ttcgggcgga ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc 3841 cttaaatggc gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat 3901 cggctcaaac atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt 3961 cgcaccttcc ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa 4021 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc 4081 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa 4141 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg 4201 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt 4261 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg 4321 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc 4381 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc 4441 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg 4501 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag 4561 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa 4621 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat 4681 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag 4741 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta 4801 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg 4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 5041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 5701 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 5941 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc 6001 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca 6061 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta 6121 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg 6181 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct 6241 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg 6301 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga 6361 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta 6421 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc 6481 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc 6541 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag 6601 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag 6661 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc 6721 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac 6781 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg 6841 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg 6901 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc 6961 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc 7021 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg 7081 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct 7141 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg 7201 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa 7261 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc 7321 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt 7381 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg 7441 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg 7501 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta 7561 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt 7621 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat 7681 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg 7741 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg 7801 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt 7861 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat 7921 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt 7981 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca 8041 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa 8101 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc 8161 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata 8221 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca 8281 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg 8341 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt 8401 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat 8461 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc 8521 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt 8581 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca 8641 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc 8701 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc 8761 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa 8821 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc 8881 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct aggcaccgcg ccgcctggcc 8941 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg 9001 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg 9061 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg 9121 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg 9181 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct 9241 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg 9301 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg 9361 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa 9421 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg 9481 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa 9541 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa 9601 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg 9661 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt 9721 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga 9781 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt 9841 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg 9901 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac 9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 10021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 10081 acgacggcca gtgccaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct 10141 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc 10201 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga 10261 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca 10321 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct 10381 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca 10441 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt 10501 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt 10561 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta 10621 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga 10681 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca 10741 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc 10801 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt 10861 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg 10921 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa 10981 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca 11041 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct 11101 cgtcctcccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag 11161 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 11221 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 11281 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 11341 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 11401 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 11461 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc tgtttcaaac 11521 tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 11581 gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 11641 ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 11701 gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 11761 ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 11821 gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 11881 catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 11941 taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 12001 cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 12061 ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtcga ctctagagga 12121 tcccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagctga acgagaaacg 12181 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 12241 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta 12301 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt 12361 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 12421 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 12481 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 12541 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 12601 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 12661 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 12721 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 12781 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 12841 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 12901 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 12961 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 13021 agtggcaggc ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc 13081 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 13141 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat 13201 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 13261 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 13321 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa 13381 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 13441 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 13501 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 13561 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 13621 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 13681 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat 13741 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 13801 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttcgataatt 13861 cttaattaac tagttctaga gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt 13921 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt 13981 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg 14041 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata 14101 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta 14161 ctagatcggg aatt //
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